Study on the Physico-chemical Factors Augmenting the Growth of Mycoplasma mycoides subspecies capri

Different physical factors like aeration, temperature and shaking culture conditions were applied to the culture and observation of growth was studied for 72 hours of incubation. Similarly the Mycoplasma mycoides subspecies capri was grown in PPLO medium enriched with different concentrations (5, 10 and 20%) of serum from horse, bovine and allantoic fluid, along with usual concentration of yeast extract (1%) to record their effect on the growth. It was observed that growth was optimum (2.7×10/ml) at 37°C in stable cultures under microaerophilic condition after 48 hours of incubation. The horse serum (20%) was suggested as an excellent biological material for enhancing the growth and replication of Mycoplasma mycoides subspecies capri (PG3). A direct positive correlation (r=0.94) was found between viable count and different protein concentrations of washed suspension of Mycoplasma mycoides subspecies capri.